Pretreatment with abscisic acid accompanied by sucrose improves callus survival after cryopreservation of hazel (Corylus avellana L.) by desiccation
نویسندگان
چکیده
In the current study, a simple cryopreservation method (desiccation) was applied to Corylus avellana L. callus. Accordingly, effects of abscisic acid (ABA) concentration, pretreatment duration on MS medium containing ABA + 10% sucrose, and storage length in liquid nitrogen (LN) callus survival rate were investigated. Calli’s assessed 8 weeks after exiting from LN. Callus significantly affected by concentration treatment with ABA-supplemented media. However, LN did not have an impact rate. present highest (45.79%) obtained both treatments 20 days preculture 2 mg l− 1 following h desiccation-one day 2.5 desiccation-thirty The desiccation help sucrose effective successful
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ژورنال
عنوان ژورنال: Plant Cell Tissue and Organ Culture
سال: 2022
ISSN: ['1573-5044', '0167-6857']
DOI: https://doi.org/10.1007/s11240-022-02401-6